rea name origin的問題，透過圖書和論文來找解法和答案更準確安心。 我們找到下列線上看、影評和彩蛋懶人包

Lactobacillus paracasei RM081 之體外益生菌特性評估

為了解決rea name origin的問題，作者貝利坎 這樣論述：

Probiotics are living microorganisms that, when administered in adequate amounts, confer a health benefit on the host. So far, many probiotic strains have been obtained from different sources, and some of them have reached commercial usage in the markets successfully. The isolation of new potential

probiotic strains has also become an increasingly important issue. Lactobacillus (L.) paracasei RM081, which is able to produce anti-inflammatory tryptophan derivatives 5-methoxytryptophan (5-MTP), was isolated from raw milk and identified by using 16s rRNA sequencing and whole-genome sequencing (W

GS). The aim of current study was to evaluate the in vitro probiotic characteristics of L. paracasei RM081 for providing an overview property of the probiotic strain for the reference of further applications. The carbohydrate metabolism profile of L. paracasei RM081 was determined by API 50 CH testi

ng. The anti-oxidative ability of the strain was evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging assay. Further, various in vitro environmental condition experiments were performed to determine its resistance to imitated gastrointestinal tract (GIT) environment or cul

ture conditions, including temperature, acid, sodium chloride (NaCl), and bile salt. In addition, L. paracasei RM081 adhering ability to intestine epithelial cell (IEC) was evaluated by auto-aggregation assay, hydrophobicity assay, and IEC human colon intestinal cell line (Caco-2) adhesion experimen

t. Its safety property was finally determined by antibiotic susceptibility testing. Our results showed that this strain has fermented some different types of carbohydrates by API 50 CH testing. Besides, a highly significant anti-oxidative ability of L. paracasei RM081 in DPPH scavenging was shown by

its cell-free supernatants compared to live cells and cell-free extracts. The incubation temperatures tested significantly increase the growth of L. paracasei RM081 (30, 37, 42 oC). But, 15 oC was not as significant as the others. The strain also survived under the imitated GIT pH (1.5, 2.5, 3.5, a

nd 4.5) and bile salt (0.1, 0.3, 0.5%) conditions, whereas the bacteria''s resistance to stress conditions was altered by dose-dependent manner. On the other hand, L. paracasei RM081 could survive in different NaCl concentrations (2, 4, 7, 10%), but the proliferation rate was reduced at 10 and 7% Na

Cl concentrations. Auto-aggregation assay, hydrophobicity assay, and Caco-2 cell adhesion experiments demonstrated that L. paracasei RM081 strain is stable in GIT and have high adherent potential to IEC (85+0.7%; 71.5+2.4%; 78+4.8%; respectively). Besides, antibiotic resistance test showed that L. p

aracasei RM081 was widely sensitive to most antibiotics but not to streptomycin. In conclusion, our results suggest that L. paracasei RM081 represents a desirable probiotic characteristic in vitro. Overall, evaluating probiotic properties of a strain isolated from raw milk is a promising approach to

prepare functional microbial cultures, and L. paracasei RM081 demonstrated to be a beneficial probiotic based on our study.

蘇氏海木耳水萃物對海鱺免疫調控及抗發光桿菌之研究

為了解決rea name origin的問題，作者邱柏諭 這樣論述：

本研究係利用蘇式海木耳水萃物 (Sarcodia suiae water extact, SSWE)進行體外和體內實驗，藉由即時定量聚合酶連鎖反應 (Quantitative real-time polymerase chain reaction, qRT-PCR)，以分析處理後之不同時間點免疫相關基因的表現。發現在初代培養之頭腎細胞中經SSWE的處理可以顯著增加細胞因子如介白素-1β、-6、-10、-12、腫瘤壞死因子-α及趨化因子介白素-8之表現，且在腹腔注射SSWE的魚隻，其免疫相關組織中發現可誘導介白素-8、-12、趨化因子CC1及免疫球蛋白T的表現。而在先腹腔注射給予SSWE，再以

不同劑量之發光桿菌 (Photobacterium damselae subsp. damselae)攻擊後發現SSWE具延遲海鱺死亡之現象。最後，腹腔注射SSWE處理後以較低濃度發光桿菌感染魚隻，發現可顯著誘發魚隻之介白素-6、-8、-12、腫瘤壞死因子α及干擾素-γ之表現量。綜合以上結果得知，蘇式海木耳水萃物具有成為免疫調節物及疫苗佐劑之潛力。