Uninvolved 醫學 中文的問題，透過圖書和論文來找解法和答案更準確安心。 我們找到下列線上看、影評和彩蛋懶人包

探討金黃色葡萄球菌在角質細胞調控絲聚蛋白之機轉

為了解決Uninvolved 醫學 中文的問題，作者蔡彥郁 這樣論述：

Abstract (中文)Abstract (English)AcknowledgementsTable of figuresTable of tablesTable of abbreviationsChapter 1: Introduction1.1Research motivation1.2 Chronic Kidney Disease1.2.1 EpidemiologyI. Prevalence globallyII. Prevalence in Taiwan1.2.2 Treatments1.2.3 Life quality of HD patients1.3 Prur

itus1.3.1 Pruritus definition1.3.2 Prevalence in hemodialysis patients1.3.3 Questionnaire1.3.4 Diagnosis and treatmentI. DiagnosisII. Treatment1.4 Skin barrier function1.4.1 Skin: an overview1.4.2 Importance of the skin barrier function1.5 Filaggrin1.5.1 Filaggrin: an overview1.5.2 The t

ransition of filaggrin to natural moisturizingfactors (NMFs) and its importance1.5.3 Signaling pathways related to filaggrin regulationI. Aryl hydrocarbon receptor (AhR)II. Ovo like protein 1 (OVOL1)III. Serine/Threonine kinase Akt (protein kinase B,PKB)IV. Mitogen-activated protein kinase/Extr

acellular signal-regulated kinases (MAPK/ERK)1.6 Staphylococcus aureus1.6.1 Staphylococcus aureus: an overview1.6.2 Toxins (Hemolysins)I. Alpha-toxin (a-toxin, a-hemolysin)II. Beta-toxin (b-toxin, b-hemolysin,sphingomyelinase C)III. Gamma-toxin (g-toxin, g-hemolysin and PV-Leukocidin)IV. Delt

a-toxin (d-toxin, d-hemolysin, d-Lysin)1.6.3 Relevance on the skinChapter 2: Materials and methods2.1 Experimental reagents and materials2.1.1 HaCaT cell line2.1.2 Experiments2.1.3 Primers2.1.4 Plasmids2.1.5 Antibodies2.1.6 Chemical reagents and solvents2.2 Equipment2.3 Experimental flowch

art2.4 Methods2.4.1 Colonization rateI. Ethics statementII. Target patientIII. QuestionnaireIV. SamplingV. DNA extraction from swabsVI. Quantitative real-time polymerase chain reaction (qPCR)2.4.2 Pathway analysisI. Cell CultureII. MTS Cell Proliferation AssayIII. TransfectionIV. Revers

e Transcription Polymerase Chain Reaction (RT-PCR)V. Quantitative real-time Polymerase Chain Reaction (qPCR)VI. SDS-PageVII. Western blotVIII. Protein Inhibitor CocktailsIX. Statistical analysisChapter 3: Results and discussion3.1 Colonization rate3.1.1 Patient characteristics3.1.2 Staphyloc

occus aureus colonization is higher in HD patients with pruritus than HD patients without pruritus3.2 Pathway analysis3.2.1 Cellular viability of different concentrations of a-toxin3.2.2 a-toxin downregulates Filaggrin mRNA levels in HaCaT cells3.2.3 a-toxin downregulates Filaggrin protein and

other protein kinases in cultured cells3.2.4 FLG mRNA is upregulated after activation of ERK and AKT, and overexpression of OVOL1 and AhRI. Reason for overexpressionII. Activation of ERK with pCMV-MEK1III. Activation of AKT with CA-AKTIV. Overexpression of OVOL1 and AhR with pCMV-OVOL1 and pCMV

-AhRV. Overexpression results3.2.5 DiscussionChapter 4: References

人類汗腺組織中Matriptase、Prostasin與其抑制劑 HAI-1/2的表現與功能

為了解決Uninvolved 醫學 中文的問題，作者郭姿佑 這樣論述：

當環境或體內溫度升高產熱，促使汗腺分泌汗液，容易引起溼疹或造成慢性發炎皮膚疾病患者刺痛與搔癢症狀更加嚴重。汗液與汗管中含有各種蛋白酶與其內生性抑制劑，汗腺導管中含有上皮鈉離子通道 (epithelial sodium channel, ENaC)，可回收鈉離子來調控皮膚的含水量。從先前研究已知prostasin可水解表皮鈉離子通道γ次單元的抑制胜肽，促使ENaC打開，而 prostasin又受 matriptase的水解而活化，並受到內生性抑制劑：第一型及第二型肝細胞生長因子活化酶抑制蛋白 (hepatocyte growth factor activator inhibitor 1/2,

HAl-1/2)的抑制。另外，異位性皮膚炎 (atopic dermatitis, AD)是一種會反覆發作的慢性發炎皮膚疾病，過去有研究提出汗液與異位性皮膚炎具關聯性。因此，本研究透過免疫組織化學染色觀察人類皮膚汗腺组織中prostasin、matriptase、HAI-1以及HAI-2的分佈，探討與ENaC之間的關係，並利用白血球介白素-4 (Interleukin-4, IL-4)、白血球介白素-13 (Interleukin -13, IL-13) 及 腫瘤壞死因子 (tumor necrosis factor-α, TNF-α) 誘導HaCaT細胞成為類異位性皮膚炎細胞模型，觀察在不同

鈉離子濃度下，蛋白酶與其內生性抑制劑表現量。本研究根據細胞型態及功能，將汗腺分成三大部分：出汗口、導管部以及分泌部。發現Total matriptase及HAI-1在汗腺導管部及分泌部都表現較強的訊號，觀察到些許活化態matriptase在分泌部；而prostasin會特別集中表在汗腺導管面向管腔之內側。在細胞實驗中，發現環境中鈉離子含量提高會使HaCaT細胞內胸腺基質淋巴生成素 (thymic stromal lymphopoietin, TSLP) 表現量增加，也發現不論在低或高鈉離子環境中，皆可以觀察到再加IL-4、IL-13及TNF-α後，prostasin、matriptase、HA

I-1與HAI-2的表現量皆改變。另外，透過細胞免疫螢光染色，發現將細胞於短時間培養於高鈉離子環境中，prostasin會逐漸從細胞核旁向細胞膜靠近。本論文中，我們發現環境中鈉離子含量高低及促發炎細胞因子，皆會影響細胞內蛋白質表現，表示汗液中的鈉離子與異位性皮膚炎的致病機制可能具有密切關係，但考慮到汗液組成複雜，未來可以進一步模擬汗液的培養環境，繼續觀察上述各種蛋白條件之變化。總體而言，環境中鈉離子濃度及細胞因子皆會影響蛋白酶與其內生性抑制劑的表現量，但其中詳細機制需要繼續探討。